RE1106_AccB1l_Gel

Vivantis

AccB1 I {HgiCI}

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Price

£15.14

CATALOGUE NO. DESCRIPTION PRICE
Re1106

AccB1I, 500 units

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Concentration
5-10u/μl

5’…G↓GYRCC…3′
3’…CCATG↑G…5′

Reaction Conditions
1X Buffer AccBII

10mM Tris-HCl (pH 7.5 at 25°C), 10mM MgCl2, 100mM KCl, and 100μg/ml BSA. Incubate at 37°C.

Storage Buffer
10mM Tris-HCl (pH 7.5), 50mM KCl, 0.1mM EDTA, 7mM 2-mercaptoethanol, 200μg/ml BSA and 50% glycerol. Store at -20°C.

Thermal Inactivation
65°C for 20 minutes.

Ligation / Recutting Assay
After 10-fold overdigestion with AccB1I, more than 95% of the DNA fragments can be ligated and recut.

Overdigestion Assay
An unaltered banding pattern was observed after 1μg of DNA was digested with 10u of AccB1I for 16 hours at 37°C.

Supplied with 10X Buffer AccB1I, 10X Buffer UB and Viva Buffer A (Diluent)

*High enzyme concentration may result in Star Activity

λDNA 1% Agarose
a – Digestion after 1 hour
b – Digestion after 16 hours

 

Activity in Reaction Buffer
V1 V2 V3 V4 V5
75% 75% 50% 50% 75%

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Manual

AccB1 I {HgiCI}

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CATALOGUE NO. DESCRIPTION PRICE
Re1106

AccB1I, 500 units

Request A Quote

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