1X Buffer AccBII
10mM Tris-HCl (pH 7.5 at 25°C), 10mM MgCl2, 100mM KCl, and 100μg/ml BSA. Incubate at 37°C.
10mM Tris-HCl (pH 7.5), 50mM KCl, 0.1mM EDTA, 7mM 2-mercaptoethanol, 200μg/ml BSA and 50% glycerol. Store at -20°C.
65°C for 20 minutes.
Ligation / Recutting Assay
After 10-fold overdigestion with AccB1I, more than 95% of the DNA fragments can be ligated and recut.
An unaltered banding pattern was observed after 1μg of DNA was digested with 10u of AccB1I for 16 hours at 37°C.
Supplied with 10X Buffer AccB1I, 10X Buffer UB and Viva Buffer A (Diluent)
*High enzyme concentration may result in Star Activity
|λDNA 1% Agarose
a – Digestion after 1 hour
b – Digestion after 16 hours
|Activity in Reaction Buffer|