RE1108_AccB7l_Gel

Vivantis

AccB7 I {PflMI}

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Price

£15.66

CATALOGUE NO. DESCRIPTION PRICE
Re1108

AccB7I, 100 units

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Concentration
5u/μl

5’… CCANNNN↓NTGG…3′
3’…GGTN↑NNNNACC…5

Reaction Conditions
1X Buffer V2

10mM Tris-HCl (pH7.5 at 30°C), 10mM MgCl2, 50mM NaCl, and 100μg /ml BSA. Incubate at 37°C.

Storage Buffer
10mM Tris-HCl (pH 7.5), 50mM KCl, 0.1mM EDTA, 7mM 2-mercaptoethanol, 200μg/ml BSA and 50% glycerol. Store at -20°C.

Thermal Inactivation
65°C for 20 minutes.

Ligation / Recutting Assay
After 5-fold overdigestion with AccB7 I, 95% of the DNA fragments can be ligated and recut.

Overdigestion Assay
An unaltered banding pattern was observed after 1μg of DNA was digested with 2.5u of AccB7I for 16 hours at 37°C.

Supplied with 10X Buffer V2, 10X Buffer UB and Viva Buffer A. (Diluent)

High enzyme concentration may result in Star Activity.

*Blocked by overlapping dcmmethylation (CmCWGG): CCANNNCCTGG or CCAGGNNNTGG

?DNA (Hind III Digest) 0.7% Agarose
a – Digestion after 1 hour
b – Digestion after 16 hours

 

Activity in Reaction Buffer
V1 V2 V3 V4 V5
25% 100% 100% 100% 75%

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Manual

AccB7 I {PflMI}

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CATALOGUE NO. DESCRIPTION PRICE
Re1108

AccB7I, 100 units

Request A Quote

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