1X Buffer V2
10mM Tris-HCl (pH7.5 at 30°C), 10mM MgCl2, 50mM NaCl, and 100μg /ml BSA. Incubate at 37°C.
10mM Tris-HCl (pH 7.5), 50mM KCl, 0.1mM EDTA, 7mM 2-mercaptoethanol, 200μg/ml BSA and 50% glycerol. Store at -20°C.
65°C for 20 minutes.
Ligation / Recutting Assay
After 5-fold overdigestion with AccB7 I, 95% of the DNA fragments can be ligated and recut.
An unaltered banding pattern was observed after 1μg of DNA was digested with 2.5u of AccB7I for 16 hours at 37°C.
Supplied with 10X Buffer V2, 10X Buffer UB and Viva Buffer A. (Diluent)
High enzyme concentration may result in Star Activity.
*Blocked by overlapping dcmmethylation (CmCWGG): CCANNNCCTGG or CCAGGNNNTGG
|?DNA (Hind III Digest) 0.7% Agarose
a – Digestion after 1 hour
b – Digestion after 16 hours
|Activity in Reaction Buffer|