RE1110_AccBSl_Gel

Vivantis

AccBSI {BsrBI}

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Price

£55.26

CATALOGUE NO. DESCRIPTION PRICE
Re1110

AccBSI, 500 units

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Concentration
5-20u/μl

5’…CCG↓CTC…3′
3’…GGC↑GAG…5′

Reaction Conditions
1X Buffer V5

30mM Tris-acetate (pH 7.9 at 30°C), 10mM Mg-acetate, 60mM K-acetate, and 100μg/ml BSA. Incubate at 37°C.

Storage Buffer
10mM Tris-HCl (pH 7.5), 100mM NaCl, 0.1mM EDTA, 7mM 2-mercaptoethanol, 200μg/ml BSA and 50% glycerol. Store at -20°C.

Thermal Inactivation
65°C for 20 minutes

Ligation / Recutting Assay
After 5-fold overdigestion with AccBSI, 90% of the DNA fragments can be ligated and of these 50% can be recut.

Overdigestion Assay
An unaltered banding pattern was observed after 1μg of DNA was digested with 10u of AccBSI for 16 hours at 37°C.

Supplied with 10X Buffer V5, 10X Buffer UB and Viva Buffer A. (Diluent)

λDNA 1.4% Agarose
a – Digestion after 1 hour
b – Digestion after 16 hours

 

Activity in Reaction Buffer
V1 V2 V3 V4 V5
100% 100% 75% 75% 100%

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Manual

AccB7 I {PflMI}

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CATALOGUE NO. DESCRIPTION PRICE
Re1110

AccBSI, 500 units

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