RE1112_Acll_Gel

Vivantis

Acl I

Email a Friend






Price

£41.54

CATALOGUE NO. DESCRIPTION PRICE
Re1112

AclI, 100 units

Request A Quote

Concentration
1-3u/μl

5’…AA↓CGTT…3′
3’…TTGC↑AA…5′

Reaction Conditions
1X Buffer V5

30mM Tris-acetate (pH7.9 at 30°C), 10mM Mg-acetate, 60mM K-acetate, and 100μg/ml BSA. Incubate at 37°C.

Storage Buffer
10mM Tris-HCl (pH 7.5), 100mM NaCl, 0.1mM EDTA, 7mM 2-mercaptoethanol, 0.05% Triton X-100, 200μg/ml BSA and 50% glycerol. Store at -20°C.

Thermal Inactivation
65°C for 20 minutes.

Ligation / Recutting Assay
After 3-fold overdigestion with Acl I, 90% of the DNA fragments can be ligated and recut.

Overdigestion Assay
An unaltered banding pattern was observed after 1μg of DNA was digested with 1.5u of Acl I for 16 hours at 37°C.

Supplied with 10X Buffer V5, 10X Buffer UB and Viva Buffer A. (Diluent)

*Blocked by CpG methylation

λDNA 0.7% Agarose
a – Digestion after 1 hour
b – Digestion after 16 hours

 

Activity in Reaction Buffer
V1 V2 V3 V4 V5
75% 75% 50% 75% 100%

DOWNLOAD

Manual

Acl I

Please Select Your Order Info

CATALOGUE NO. DESCRIPTION PRICE
Re1112

AclI, 100 units

Request A Quote

Reviews

There are no reviews yet.

Be the first to review “Acl I”

Your email address will not be published. Required fields are marked *

(0 Items)
0
View cart

SECURED TRANSACTION