1X Buffer V5
30mM Tris-acetate (pH7.9 at 30°C), 10mM Mg-acetate, 60mM K-acetate, and 100μg/ml BSA. Incubate at 37°C.
10mM Tris-HCl (pH 7.5), 100mM NaCl, 0.1mM EDTA, 7mM 2-mercaptoethanol, 0.05% Triton X-100, 200μg/ml BSA and 50% glycerol. Store at -20°C.
65°C for 20 minutes.
Ligation / Recutting Assay
After 3-fold overdigestion with Acl I, 90% of the DNA fragments can be ligated and recut.
An unaltered banding pattern was observed after 1μg of DNA was digested with 1.5u of Acl I for 16 hours at 37°C.
Supplied with 10X Buffer V5, 10X Buffer UB and Viva Buffer A. (Diluent)
*Blocked by CpG methylation
|λDNA 0.7% Agarose
a – Digestion after 1 hour
b – Digestion after 16 hours
|Activity in Reaction Buffer|