1X Buffer Ama87I
10mM Tris-HCl (pH8.5 at 30°C), 10mM MgCl2 , 100mM KCl, and 100μg/ml BSA. Incubate at 37°C.
10mM KH2PO4 (pH 7.2), 100mM NaCl, 0.1mM EDTA, 7mM 2-mercaptoethanol, and 50% glycerol. Store at -20°C.
65°C for 20 minutes.
Ligation / Recutting Assay
After 10-fold overdigestion with Ama87I, more than 90% of the DNA fragments can be ligated and recut.
An unaltered banding pattern was observed after 1μg of DNA was digested with 20u of Ama87I for 16 hours at 37°C.
Supplied with 10X Buffer Ama87I, 10X Buffer UB and Viva Buffer A. (Diluent)
High enzyme contraction may result in Star Activity.
|λDNA 0.7% Agarose
a – Digestion after 1 hour
b – Digestion after 16 hours
|Activity in Reaction Buffer|