RE1122_Ama87l_Gel

Vivantis

Ama87I {AvaI}

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Price

£40.66

CATALOGUE NO. DESCRIPTION PRICE
Re1122

Ama87I, 600 units

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Concentration
10-30u/μl

5’…C↓YCGRG…3′
3’…GRGCY↑C…5′

Reaction Conditions
1X Buffer Ama87I

10mM Tris-HCl (pH8.5 at 30°C), 10mM MgCl2 , 100mM KCl, and 100μg/ml BSA. Incubate at 37°C.

Storage Buffer
10mM KH2PO4 (pH 7.2), 100mM NaCl, 0.1mM EDTA, 7mM 2-mercaptoethanol, and 50% glycerol. Store at -20°C.

Thermal Inactivation
65°C for 20 minutes.

Ligation / Recutting Assay
After 10-fold overdigestion with Ama87I, more than 90% of the DNA fragments can be ligated and recut.

Overdigestion Assay
An unaltered banding pattern was observed after 1μg of DNA was digested with 20u of Ama87I for 16 hours at 37°C.

Supplied with 10X Buffer Ama87I, 10X Buffer UB and Viva Buffer A. (Diluent)

High enzyme contraction may result in Star Activity.

λDNA 0.7% Agarose
a – Digestion after 1 hour
b – Digestion after 16 hours

 

Activity in Reaction Buffer
V1 V2 V3 V4 V5
10% 25% 50% 75% 10%

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Manual

Ahl I {SpeI}

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CATALOGUE NO. DESCRIPTION PRICE
Re1122

Ama87I, 600 units

Request A Quote

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