RV1138_BamHl_Gel

Vivantis

BamHI

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Price

£24.82

CATALOGUE NO. DESCRIPTION PRICE
Rv1138

BamHI, 2500 units

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Concentration
40-150u/μl

5’…G↓GATCC…3′
3’…CCTAG↑G…5′

Reaction Conditions
1X Buffer UB

25mM Tris-acetate (pH7.6 at 30°C), 10mM Mg-acetate, 100mM K-acetate, 7mM 2-mercaptoethanol, and 50μg/ml BSA. Incubate at 37°C.

Storage Buffer
10mM Tris-HCl (pH 7.5), 200mM NaCl, 0.1mM EDTA, 1mM DTT, 0.15% Triton X-100, 200μg/ml BSA and 50% glycerol. Store at -20°C.

Thermal Inactivation
65°C for 20 minutes.

Ligation / Recutting Assay
After 40-fold overdigestion with BamHI, more than 90% of the DNA fragments can be ligated and recut.

Overdigestion Assay
An unaltered banding pattern was observed after 1μg of DNA was digested with 10u of BamHI for 16 hours at 37°C.

Supplied with 10X Buffer UB and Viva Buffer A. (Diluent)

*High enzyme concentration may result in Star Activity.

λDNA 0.7% Agarose
a – Digestion after 1 hour
b – Digestion after 16 hours

 

Activity in Reaction Buffer
V1 V2 V3 V4 V5
75% 75% 50% 75% 50%

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Manual

BamHI

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CATALOGUE NO. DESCRIPTION PRICE
Rv1138

BamHI, 2500 units

Request A Quote

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