RE1140_Bbv12l_Gel

Vivantis

Bbv12l {HgiAI}

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Price

£15.66

CATALOGUE NO. DESCRIPTION PRICE
Re1140

Bbv12I, 100 units

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Concentration
1-5u/μl

5’…GCCNNNN↓NGGC…3′
3’…CGGN↑NNNNCCG…5′

Reaction Conditions
1X Buffer Bbv12I

20mM Tris-HCl (pH8.5), 10mM MgCl2, 20mM NaCl, and 1mM DTT. Incubate at 37°C.

Storage Buffer
10mM Tris-HCl (pH 7.5), 100mM NaCl, 0.1mM EDTA, 7mM 2-mercaptoethanol, 200μg/ml BSA and 50% glycerol. Store at -20°C.

Thermal Inactivation
None.

Ligation / Recutting Assay
After 5-fold overdigestion with Bbv12I, more than 90% of the DNA fragments can be ligated and recut.

Overdigestion Assay
An unaltered banding pattern was observed after 1μg of DNA was digested with 5u of Bbv12I for 16 hours at 37°C.

Supplied with 10X Buffer V3, 10X Buffer UB and Viva Buffer A. (Diluent)

λDNA 0.7% Agarose
a – Digestion after 1 hour
b – Digestion after 16 hours

 

Activity in Reaction Buffer
V1 V2 V3 V4 V5
10% 25% 75% 25% 25%

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Manual

Bbv12l {HgiAI}

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CATALOGUE NO. DESCRIPTION PRICE
Re1140

Bbv12I, 100 units

Request A Quote

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