1X Buffer Bbv12I
20mM Tris-HCl (pH8.5), 10mM MgCl2, 20mM NaCl, and 1mM DTT. Incubate at 37°C.
10mM Tris-HCl (pH 7.5), 100mM NaCl, 0.1mM EDTA, 7mM 2-mercaptoethanol, 200μg/ml BSA and 50% glycerol. Store at -20°C.
Ligation / Recutting Assay
After 5-fold overdigestion with Bbv12I, more than 90% of the DNA fragments can be ligated and recut.
An unaltered banding pattern was observed after 1μg of DNA was digested with 5u of Bbv12I for 16 hours at 37°C.
Supplied with 10X Buffer V3, 10X Buffer UB and Viva Buffer A. (Diluent)
|λDNA 0.7% Agarose
a – Digestion after 1 hour
b – Digestion after 16 hours
|Activity in Reaction Buffer|