The GF-1 Bacterial DNA Extraction Kit provides a rapid and efficient method for purification of high molecular weight genomic DNA from either Gram-negative or Gram-positive bacteria. The purification is based on the usage of denaturing agents to provide lysis of cells, denaturation of proteins and subsequently release of genomic DNA. Special buffers provided in the kit are optimized to enhance the binding of DNA onto a specially-treated glass filter membrane for efficient recovery of highly pure genomic DNA.
- Suitable for both Gram-negative or Gram-positive bacteria
- Yields up to 20μg of DNA
- No organic-based extraction required
- Highly pure genomic DNA ready to use for routine molecular biology applications such as restriction enzyme digestion, PCR, Southern blotting and DNA fingerprinting.
- Buffer R1
- Buffer R2
- Buffer BG
- Wash Buffer (concentrate)
- Elution Buffer
- Proteinase K