Taq DNA Polymerase is a thermostable DNA polymerase. It is suitable for applications requiring high temperature synthesis of DNA. Taq DNA Polymerase catalyzes the polymerization of nucleotides into duplex DNA in the 5′ to 3′ direction with the presence of Mg2+ and has the 5′ to 3’exonuclease activity.
- Ultra pure recombinant protein allows amplification up to 8kb.
- 10X ViBuffer S provided for amplification of more than 5kb amplicon.
- Generates mostly 3′ dA overhang amplification products which are suitable for TA cloning.
1u is defined as the amount of enzyme that is required to catalyze the incorporation of 10nmol of dNTP into acid-insoluble material in 30 minutes at 74°C. The reaction conditions are: 50mM Tris-HCl (pH 9.0 at 25°C), 50mM NaCl, 5mM MgCl2, 200μM each of dATP, dCTP, dGTP, dTTP (a mix of unlabeled and [³H]dTTP), 10μg activated calf thymus DNA and 100μg/ ml BSA in a final volume of 50μl.
- 10X ViBuffer A (without MgCl2)
500mM KCl, 100mM Tris-HCl (pH 9.1 at 20°C) and 0.1% Triton™ X-100. The buffer is optimized for use with 0.1-0.2mM of each dNTP
- 10X ViBuffer S
160mM (NH4)2SO4, 500mM TrisHCl (pH 9.2 at 22°C), 17.5mM MgCl2 and 0.1% Triton™ X-100. The buffer is optimized for use with 0.35mM of each dNTP.
- 50mM MgCl2
All preparations are assayed for contaminating endonuclease, exonuclease, and non-specific DNase activities. Functionally tested in DNA amplification.
20mM Tris-HCl (pH 8.0 at 22°C), 100mM KCl, 0.5% Tween™ 20, 0.5% Nonidet-P40, 0.1mM EDTA, 1mM DTT, color dyes and 50% glycerol. Store at -20°C.
|Amplification Using Vivantis Taq DNA Polymerase|
|Lane M1: VC 1kb DNA Ladder
Lane 1 : 1.5kb amplicon
Lane 2 : 5kb amplicon
Lane 3 : 8kb amplicon
Lane 4 : 15kb amplicon
Lane 5 : 20kb amplicon
Lane M2 : VC Lamba / Hind III Marker