ViSafe Green Gel Stain is a stable, sensitive and environmentally safe fluorescent nucleic acid dye for staining double-stranded DNA (dsDNA), single- stranded DNA (ssDNA) or RNA in agarose gels or polyacrylamide gels.
ViSafe Green Gel Stain has UV absorption between 250nm and 300nm. It is compatible with either 254nm UV transilluminator or a gel reader equipped with visible excitation. Both green gel stain and EtBr have the same spectra, so the green gel stain able to replace Ethidium Bromide (EtBr) without changing existing imaging system.
The green gel stain is designed to replace the highly toxic ethidium bromide (EtBr). The dye is confirmed by Ames test results that it is impenetrable to latex gloves and cell membranes. By using the suggested working concentrations in gel staining, the dye is proven unable to cross cell membranes; and it is noncytotoxic and nonmutagenic at working concentrations.
ViSafe Green Gel Stain, 10000X in H2O, can be diluted 10000X for precast gel protocol or 3000-3300X for post gel staining. One vial (0.5ml) of 10000X solution can be used for at least 100 minigels either using precast method or post-staining method.
Stable at room temperature for long-term storage. Stable to be microwaved or being heated. The working solution is stable to be at room temperature and kept in dark
The stain is noncytotoxic & nonmutagenic shown by Ames tests.
- Higher sensitivity
More sensitive compared to EtBr or SybrGreen 10000X gel staining dye.
- Extremely stable
- Wide application
Suitable to stain dsDNA, ssDNA and RNA. Suitable to use in agarose gel or polyacrylamide gel. Compatible with downstream applications, such as gel recovery, cloning.
- Easy staining protocols
Easy precast gel staining & post-staining procedures.
- Compatible with most imaging system
Gel can be viewed with standard UV transilluminator, visible light gel reader, or other gel imaging system.
Shipped at ambient temperature. Stored at 2-8℃ or -20℃
Recommended Equipment Required But Not Supplied:
- ElitePro Single Channel Adjustable Volume Pipette, 1-10uL
(Hercuvan Cat No: TT-EP-10U)
- MiniGEL Electrophoresis System
(Hercuvan Cat No: TT-HES-1-110V/TT-HES-1-220V)
- SafeViewer Blue Light Transilluminator
(Hercuvan Cat No: TT-BLT-470)
|Post-staining for Agarose Gel
Figure 1: Various ladders and makers run at 1.5% TBE agarose gel. The agarose gel is post-stained with ViSafe Green Gel Stain. The gel is visualized using transilluminator with (a) blue light; (b) UV light
|Precast for Agarose Gel
Figure 2: The agarose gel is pre-stained with ViSafe Green Gel Stain. Various ladders and makers run at 1.5% TBE pre-stained agarose gel. The gel is visualized using transilluminator with (a) blue light; (b) UV light