ViSafe Red Gel Stain is a stable, sensitive and environmentally safe fluorescent nucleic acid dye for staining double stranded DNA (dsDNA), single stranded DNA (ssDNA) or RNA in agarose gels or polyacrylamide gels.
ViSafe Red Gel Stain is designed to replace the highly toxic ethidium bromide (EtBr). Both red gel stain and EtBr have the same spectra, so the red gel stain able to replace the EtBr without changing existing imaging system. The dye is confirmed by Ames test results that it is impenetrable to latex gloves and cell membranes. By using the suggested working concentrations in gel staining, the red gel stain is proven unable to cross cell membranes; and it is noncytotoxic and nonmutagenic at working concentrations.
ViSafe Red Gel Stain, 10000X in H2O, can be diluted 10000X for precast gel protocol or 3000-3300X for post gel staining. One vial (0.5ml) of 10000X solution can be used for at least 100 minigels either using precast method or poststaining method.
The red gel stain is non cytotoxic & non mutagenic shown by Ames tests.
More sensitive compared to EtBr or Viva SybrGreen Nucleic Acid Stain.
Stable at room temperature for long-term storage.Stable to be microwaved or being heated. The working solution is stable at room temperature when kept in dark.
Suitable to stain dsDNA, ssDNA and RNA. Suitable to use in agarose gel or polyacrylamide gel. Compatible with downstream applications, such as gel recovery & cloning.
Easy staining protocols
Easy precast gel staining & post-staining procedures.
Compatible with most imaging system
Gel can be viewed with standard UV transilluminator, visible light gel reader, or other gel imaging system.
Shipped at ambient temperature Stored at 2-8℃ or -20℃
Recommended Equipment Required But Not Supplied:
- ElitePro Single Channel Adjustable Volume Pipette, 1-10uL
(Hercuvan Cat No: TT-EP-10U)
- MiniGEL Electrophoresis System
(Hercuvan Cat No: TT-HES-1-110V/TT-HES-1-220V)
- SafeViewer Blue Light Transilluminator
(Hercuvan Cat No: TT-BLT-470)
|Post-staining for Agarose Gel
Figure 1: Various ladders and makers run at 1.5% TBE agarose gel. The agarose gel is post-stained with ViSafe Red Gel Stain. The gel is visualized using transilluminator with (a) blue light; (b) UV light
|Precast for Agarose Gel
Figure 2: The agarose gel is pre-stained with ViSafe Green Gel Stain. Various ladders and makers run at 1.5% TBE pre-stained agarose gel. The gel is visualized using transilluminator with (a) blue light; (b) UV light.